DNA Extraction and Purification
Takara Bio NucleoSpin Gel and PCR Clean-Up Columns | 250 Columns
NucleoSpin Gel and PCR Clean-up Columns (250) binding columns for gel extraction and PCR clean-up.
Zymo Research Corporation DNA CLEAN&CONCENTRATOR MAGBEAD
The DNA Clean & Concentrator MagBead Kit is magnetic bead based clean-up kit that can purify DNA from PCR, enzymatic reactions, impure extractions, and other sources. The MagBinding Bead technology provides a rapid and scalable workflow that can be adapted for low to high-throughput automated method for purification and concentration of DNA.
Zymo Research Corporation Urine Conditioning Buffer (8 ml)
Features- Effectively preserves DNA and RNA in urine atambient temperatures- Facilitates pelleting of both cellular and cell-freenucleic acids from large volume urine samples- Inhibits microbial growth during long-term (cold-free)storage of urine samples
Macherey-Nagel NucleoBond Xtra Maxi EF, 10 preps
NucleoBond Xtra Maxi EF (10) 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A
Zymo Research Corporation DNA Clean & Concentrator™-25 (50 Preps) w/ Zymo-Spin™ II Columns (Uncapped)
It can also be used for post-RT cDNA clean-up and purification of sequencing-ready DNA from M13 phage. Simply add the specially formulated DNA Binding Buffer to your sample and transfer the mixture to the supplied Zymo-Spin™ Column. There is no need for organic denaturants or chloroform. Instead, the product features ZymoSpin column technology to yield DNA that is free of salts and contaminants in just 2 minutes. The purified DNA is ideal for DNA ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures.
New England Biolabs, Inc. Monarch® RNase A - 1 ml - (2 x 0.5ml)
Monarch RNase A, a component of the Monarch Genomic DNA Purification Kit, can be used to purify genomic DNA from a variety of sample types. It is used at 56°C in the kit protocol but is active at any temperature 20-65°C. Treatment with RNase A is an optional step in the Monarch Genomic DNA Purification Kit protocol for removal of any residual RNA. Co-purification of RNA during gDNA extractions is a common problem that leads to the overestimation of DNA yield. The low alcohol binding conditions employed in the kit optimizes binding of gDNA alone, so RNA co-purification is extremely low.
- Monarch RNase A degrades single-stranded RNA at C and U residues
- Purified from cow pancreas and is free of contaminating protease or DNase activity
- Contains no residual host DNA
- It is supplied as a 20 mg/ml solution in 50% glycerol, 50 mM Tris-Cl pH 7.4
- Monarch kit components are also available separately for added convenience
Zymo Research Corporation Select-a-Size DNA MagBead Kit
This simple workflow allows for specific cutoffs thatcan be modified to suit reaction clean-up, left-sided, right-sided or even double-sided size selection. The Select-a-Size MagBead Buffer selectively binds fragments based on the volume added relative to the sample. Simply choose a desired cutoff to bind the target of interest onto the beads and remove species outside of this range. The desired DNA is easily eluted from the beads following a rapid wash regimen.
Macherey-Nagel Collection Tubes (1000)
Collection Tubes (1000) Collection Tubes 2 mL, without lid pack of 1000
Zymo Research Corporation ZymoPURE-Express Plasmid MidiPrep Kit (25 Preps)
The ZymoPURE - Express Plasmid Midiprep Kit utilizes a patented alkaline lysis method for purifying up to 1.2 mg of high-quality endotoxin-free plasmid DNA directly from the culture without the pelleting and resuspension steps. Just add the ZP Express Lysis Buffer directly to your bacterial culture, neutralize, filter debris, and purify using the patented Zymo-Spin V-P microcentrifuge column. The ZymoPURE - Express Plasmid Midiprep Kit is the fastest and easiest method for isolating plasmid DNA from E. coli. The innovative binding chemistry (patent pending) enables highly concentrated DNA to be eluted directly from microcentrifuge column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, and protein. The eluted plasmid DNA ideal for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
New England Biolabs, Inc. Thermolabile Proteinase K – 30 units
Thermolabile Proteinase K (TLPK) is an engineered, subtilisin-related serine protease that will hydrolyze a broad variety of peptide bonds. It preferentially cleaves the peptide bond at the carboxyl side of aliphatic or aromatic amino acid residues. TLPK can be completely inactivated by incubation at 55°C for 10 minutes, which allows for subsequent enzymatic steps in the same reaction vessel.
Macherey-Nagel NucleoBond Xtra Maxi Plus EF, 10 preps
NucleoBond Xtra Maxi Plus EF (10) 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, NucleoBond Finalizers Large, syringes, buffers, RNase A